Izvorni kod stranice PI3K

{{Pfam_box
| Symbol = PI3 PI4 kinaza
| Name = Fosfatidilinozitol 3- i 4-kinaza
| image = PI3kinase.png
| width =
| caption = PI3 Kinaza 110 gama vezana za inhibitor PIK-93 (zut).<ref name="pmid16647110">{{PDB|2chz}}; {{cite journal | author = Knight ZA, Gonzalez B, Feldman ME, Zunder ER, Goldenberg DD, Williams O, Loewith R, Stokoe D, Balla A, Toth B, Balla T, Weiss WA, Williams RL, Shokat KM | title = A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling | journal = Cell | volume = 125 | issue = 4 | pages = 733–47 | year = 2006 | month = May | pmid = 16647110 | doi = 10.1016/j.cell.2006.03.035 | url =  }}</ref>
| Pfam = PF00454
| InterPro = IPR000403
| SMART = SM00146
| PROSITE = PDOC00710
| SCOP = 3gmm
| TCDB =
| OPM family =
| OPM protein =
| PDB = {{PDB2|1cja}}, {{PDB2|1e7u}}, {{PDB2|1e7v}}, {{PDB2|1e8w}}, {{PDB2|1e8x}}, {{PDB2|1e8y}}, {{PDB2|1e8z}}, {{PDB2|1e90}}, {{PDB2|1he8}}, {{PDB2|2a4z}}, {{PDB2|2a5u}}, {{PDB2|2chw}}, {{PDB2|2chx}}, {{PDB2|2chz}}
}}
{{Infobox Enzim
| Name = Fosfatidilinozitol 3-kinaza
| EC_number = 2.7.1.137
| CAS_number = 115926-52-8
| IUBMB_EC_number = 2/7/1/137
| GO_code = 
| image = 
| width = 
| caption =
}}
'''Fosfatidilinozitol 3-kinaze''' ({{EC broj|2.7.1.137}}, ''1-fosfatidilinozitolna 3-kinaza'', ''tip III fosfoinozitidna 3-kinaza'', ''Vps34p'', ''tip I fosfatidilinozitolna kinaza'', PI 3-kinaze ili PI3K) su familija enzima koji učestvuju u ćelijskim funkcijama poput ćelijskog rasta, proliferacije, diferencijacije, motiliteta, opstanka i intracelularne razmene materije. Sistematsko ime ovog enzima je ''ATP:1-fosfatidil-1D-mio-inozitol 3-fosfotransferaza''.<ref>{{cite journal | title = Synthesis and function of 3-phosphorylated inositol lipids | url = https://archive.org/details/sim_annual-review-of-biochemistry_2001_70/page/535 |author = Vanhaesebroeck, B., Leevers, S.J., Ahmadi, K., Timms, J., Katso, R., Driscoll, P.C., Woscholski, R., Parker, P.J. and Waterfield, M.D. |journal = Annu. Rev. Biochem. |date = 2001 |volume = 70 |pages = 535-602 |pmid = 11395417}}</ref> Ovaj enzim [[Kataliza|katalizuje]] sledeću [[hemijska reakcija|hemijsku reakciju]]

: [[Adenozin-trifosfat|ATP]] + 1-fosfatidil-1D-mio-inozitol <math>\rightleftharpoons</math> [[Adenozin-difosfat|ADP]] + 1-fosfatidil-1D-mio-inozitol 3-fosfat

PI3K kinaze su familija intracelularnih [[Prenos signala (biologija)|prenosnika signala]] koji imaju sposobnost [[fosforilacija|fosforilacije]] 3 pozicije [[hidroksil]]ne grupe [[inozitol]]nog prstena [[fosfatidilinozitol]]a (PtdIns).<ref>{{cite web |url=http://www.chem.qmul.ac.uk/iupac/cyclitol/myo.html |title=Myo-inositol}}</ref> Oni su tako]e poznati kao fosfatidilinozitol-3-kinaze. Put koji sadrži [[onkogen]] [[PIK3CA]] i tumor supresor [[PTEN (gen)]] je impliciran u neosetljivost [[tumor]]a na [[insulin]] i [[Insulinu sličan faktor rasta 1|IGF1]].<ref name="urlCell pathway on overdrive prevents cancer response to dietary restriction">{{cite web | url = http://www.physorg.com/news156000728.html | title = Cell pathway on overdrive prevents cancer response to dietary restriction | author = Giese N | authorlink = | coauthors = | date = 11. 03. 2009. | work = | publisher = PhysOrg.com | pages = | language = | archiveurl = | archivedate = | quote = | accessdate = 22. 04. 2009.}}</ref><ref name="pmid19279572">{{cite journal | author = Kalaany NY, Sabatini DM | title = Tumours with PI3K activation are resistant to dietary restriction | journal = Nature | volume = 458 | issue = 7239 | pages = 725–31 | year = 2009 | month = April | pmid = 19279572 | doi = 10.1038/nature07782 | url = | issn = | pmc = 2692085 }}</ref>

== Otkriće ==
PI 3-kinaze su otkrili Luis C. Kantli i njegovi saradnici. Do otkrića je došlo nakon identifikacije prethodno nepoznate [[fosfatidilinozitol|fosfoinozitidne]] kinaze vezane za polioma srednji T protein.<ref>{{cite journal |author=Whitman M, Kaplan DR, Schaffhausen B, Cantley L, Roberts TM |title=Association of phosphatidylinositol kinase activity with polyoma middle-T competent for transformation |journal=Nature |volume=315 |issue=6016 |pages=239–42 |year=1985 |pmid=2987699 |doi= 10.1038/315239a0|url=}}</ref> Oni su primetili jedinstvenu supstratnu specifičnost i hromatografske osobine proizvoda ove lipidne kinaze, što je dovelo do otkrića ove fosfoinozitidne kinaze sa izuzetnom sposobnošću da fosforiliše [[fosfatidilinozitol|fosfoinozitide]] u 3' poziciji [[inozitol]]nog prstena.<ref name=Whitman>{{cite journal |author=Whitman M, Downes CP, Keeler M, Keller T, Cantley L |title=Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate |journal=Nature |volume=332 |issue=6165 |pages=644–6 |year=1988 |month=April |pmid=2833705 |doi=10.1038/332644a0 |url=}}</ref> Oni su naknadno pokazali da in vivo enzim preferira PtdIns(4,5)P2 kao supstrat, čime se proizvodi fosfoinozitid [[Fosfatidilinozitol (3,4,5)-trisfosfat|PtdIns(3,4,5)P3]].<ref name=Auger>{{cite journal |author=Auger KR, Serunian LA, Soltoff SP, Libby P, Cantley LC |title=PDGF-dependent tyrosine phosphorylation stimulates production of novel polyphosphoinositides in intact cells |journal=Cell |volume=57 |issue=1 |pages=167–75 |year=1989 |month=April |pmid=2467744 |doi= 10.1016/0092-8674(89)90182-7|url=}}</ref>

== Literatura ==
{{reflist|2}}

== Dodatna literatura ==
{{refbegin|2}}
* {{Cite journal |author=Vanhaesebroeck B, Leevers S, Ahmadi K, Timms J, Katso R, Driscoll P, Woscholski R, Parker P, Waterfield M |title=Synthesis and function of 3-phosphorylated inositol lipids |url=https://archive.org/details/sim_annual-review-of-biochemistry_2001_70/page/535 |journal=Annu Rev Biochem |volume=70 |issue= |pages=535–602 |year= 2001|pmid=11395417 |doi=10.1146/annurev.biochem.70.1.535}} [http://arjournals.annualreviews.org/doi/abs/10.1146%2Fannurev.biochem.70.1.535]{{Dead link|date=October 2022 |bot=InternetArchiveBot |fix-attempted=yes }}
* {{Cite journal |author=Schild C, Wirth M, Reichert M, Schmid RM, Saur D, Schneider G |title=PI3K signaling maintains [[Myc|c-myc]] expression to regulate transcription of E2F1 in pancreatic cancer cells |journal=Mol. Carcinog. |volume= 48|issue= 12|pages= 1149–58|year=2009 |month=July |pmid=19603422 |doi=10.1002/mc.20569 |url=}}
* {{Cite journal |url=http://www.biochemsoctrans.org/bst/037/0615/bst0370615.htm |title=Form and flexibility in phosphoinositide 3-kinases |first5=X |last5=Zhang |first4=WC |last4=Hon |first3=S |last3=Miller |first2=A |last2=Berndt |author=Williams et al. |year=2009 |pmid=19614567 |doi=10.1042/BST0370615 |journal=Biochemical Society Transactions |volume=37 |issue=Pt 4 |pages=615–626 }}
{{refend}}

== Vanjske veze ==
* {{MeshName|PI-3+Kinase}}
* [http://www.cellsignal.com/pathways/akt-signaling.jsp PI3K/Akt signalni put]
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{{Kinaze}}

{{Enzimi}}

[[Kategorija:EC 2.7.1]]
[[Kategorija:Onkologija]]
[[Kategorija:Enzimi sa poznatom strukturom]]