Izvorni kod stranice Piridoksal 5'-fosfatna sintaza (hidroliza glutamina)

{{Infobox Enzim
| Name = Piridoksal 5'-fosfatna sintaza (hidroliza glutamina)
| EC_number = 4.3.3.6
| CAS_number = 
| IUBMB_EC_number = 4/3/3/6
| GO_code = 
| image = 
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'''Piridoksal 5'-fosfatna sintaza (hidroliza glutamina)''' ({{EC broj|4.3.3.6}}, ''PdxST'') je [[enzim]] sa sistematskim imenom ''D-riboza 5-fosfat,D-gliceraldehid 3-fosfat piridoksal 5'-fosfat-lijaza''.<ref>{{cite journal | title = Reconstitution and biochemical characterization of a new pyridoxal-5&prime;-phosphate biosynthetic pathway |author = Burns, K.E., Xiang, Y., Kinsland, C.L., McLafferty, F.W. and Begley, T.P. |journal = J. Am. Chem. Soc. |date = 2005 |volume = 127 |pages = 3682-3683 |pmid = 15771487}}</ref><ref>{{cite journal | title = On the two components of pyridoxal 5&prime;-phosphate synthase from ''Bacillus subtilis'' |author = Raschle, T., Amrhein, N. and Fitzpatrick, T.B. |journal = J. Biol. Chem. |date = 2005 |volume = 280 |pages = 32291-32300 |pmid = 16030023}}</ref><ref>{{cite journal | title = Structure of a bacterial pyridoxal 5&prime;-phosphate synthase complex |author = Strohmeier, M., Raschle, T., Mazurkiewicz, J., Rippe, K., Sinning, I., Fitzpatrick, T.B. and Tews, I. |journal = Proc. Natl. Acad. Sci. USA |date = 2006 |volume = 103 |pages = 19284-19289 |pmid = 17159152}}</ref><ref>{{cite journal | title = Reaction mechanism of pyridoxal 5&prime;-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate |author = Raschle, T., Arigoni, D., Brunisholz, R., Rechsteiner, H., Amrhein, N. and Fitzpatrick, T.B. |journal = J. Biol. Chem. |date = 2007 |volume = 282 |pages = 6098-6105 |pmid = 17189272}}</ref><ref>{{cite journal | title = Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5&prime;-phosphate |author = Hanes, J.W., Keresztes, I. and Begley, T.P. |journal = Angew. Chem. Int. Ed. Engl. |date = 2008 |volume = 47 |pages = 2102-2105 |pmid = 18260082}}</ref><ref>{{cite journal | title = Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate |author = Hanes, J.W., Burns, K.E., Hilmey, D.G., Chatterjee, A., Dorrestein, P.C. and Begley, T.P. |journal = J. Am. Chem. Soc. |date = 2008 |volume = 130 |pages = 3043-3052 |pmid = 18271580}}</ref><ref>{{cite journal | title = <small><sup>13</sup></small>C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase |author = Hanes, J.W., Keresztes, I. and Begley, T.P. |journal = Nat. Chem. Biol. |date = 2008 |volume = 4 |pages = 425-430 |pmid = 18516049}}</ref><ref>{{cite journal | title = Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase  complex from ''Bacillus subtilis'' |author = Wallner, S., Neuwirth, M., Flicker, K., Tews, I. and Macheroux, P. |journal = Biochemistry |date = 2009 |volume = 48 |pages = 1928-1935 |pmid = 19152323}}</ref> Ovaj enzim [[Kataliza|katalizuje]] sledeću [[hemijska reakcija|hemijsku reakciju]]

: D-riboza 5-fosfat + D-gliceraldehid 3-fosfat + L-[[glutamin]] <math>\rightleftharpoons</math> piridoksal 5'-[[fosfat]] + L-[[glutamat]] + 3H<sub>2</sub>O + [[fosfat]] (sveukupna reakcija)
:(1a) L-glutamin + H<sub>2</sub>O <math>\rightleftharpoons</math> L-glutamat + NH<sub>3</sub>
:(1b) D-riboza 5-fosfat + D-gliceraldehid 3-fosfat + NH<sub>3</sub> <math>\rightleftharpoons</math> piridoksal 5'-fosfat + 4H<sub>2</sub>O + fosfat

Amonijak obezbeđuje glutaminazna podjedinica.

== Reference ==
{{reflist}}

== Literatura ==
{{refbegin|2}}
* {{PriceEnzymology3rd}}
* {{TooneAdvancesInEnzymology}}
* {{Branden ProteinStructure2nd}}
* {{SegelEnzymeKinetics}}
* {{CopelandEvaluationOfEnzymeInhibitors2nd}}
* {{MichalBiochemicalPathways}}
{{refend}}

== Vanjske veze ==
* {{MeshName|Pyridoxal+5'-phosphate+synthase+(glutamine+hydrolyzing)}}

{{Enzimi}}

[[Kategorija:EC 4.3.3]]